CD45

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone F10-89-4
Applications FC, IP, IHC
Amount 0.025 mg
ArtNr 20-783-310734
Available
Genway ID:
GWB-3D543A
Specificity:
CD45NCBI
Gene ID:
5788
Specificity:
CD45
Clone:
F10-89-4
Immunogen:
Human T lymphocytes
Fusion Partner:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Immunohistology:
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH8. 0 is recommended for this purpose. Histology: Human Tonsil
Preparation:
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/10 - 1/50Immunohistology - Frozen - 1/500 - 1/1000Immunohistology - Paraffin - 1/100 - 1/200
Function:
Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation recruits an dephosphorylates SKAP1 and FYN.
Catalytic Activity:
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit:
Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Domain:
The first PTPase domain interacts with SKAP1.
Ptm:
Heavily N- and O-glycosylated.
Disease:
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Disease:
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Similarity:
Contains 2 fibronectin type-III domains.
Similarity:
Contains 2 tyrosine-protein phosphatase domains. 1. Dalchau. R. et al. (1980) Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat 2. Quenby. S et al. (1999) Pre-implantation endometrial leukocytes in women with recurrent miscarriage.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Discount: -10.0%
Price: €137.14
You save: €15.24
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