MOUSE ANTI V5-TAG

V5-TAG

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.

Has been used to detect recombinant proteins some of which include transmembrane and secreted proteins which have been tagged with this epitope. Usually a 14 amino acid tag has been added to the recombinant proteins although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) 1. Wang X. W. and Xu B. (1998) Stimulation of tumor-cell growth by alpha-fetoprotein. Int. J. Cancer 9:596-599. [1] \" Primary structures of human alpha-fetoprotein and its mRNA. \" Morinaga T. Sakai M. Wegmann T. G. Tamaoki T. Proc. Natl. Acad. Sci. U. S. A. 80:4604-4608(1983) [PubMed: 6192439] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" Structure polymorphism and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene. \" Gibbs P. E. M. Zielinski R. Boyd C. Dugaiczyk A. Biochemistry 26:1332-1343(1987) [PubMed: 2436661] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [3] \" Complete sequencing and characterization of 21 243 full-length human cDNAs. \" Ota T. Suzuki Y. Nishikawa T. Otsuki T. Sugiyama T. Irie R. Wakamatsu A. Hayashi K. Sato H. Nagai K. Kimura K. Makita H. Sekine M. Obayashi M. Nishi T. Shibahara T. Tanaka T. Ishii S. Yamamoto J. Saito K. Kawai Y. Isono Y. Nakamura Y. Nagahari K. Murakami K. Yasuda T. Iwayanagi T. Wagatsuma M. Shiratori A. Sudo H. Hosoiri T. Kaku Y. Kodaira H. Kondo H. Sugawara M. Takahashi M. Kanda K. Yokoi T. Furuya T. Kikkawa E. Omura Y. Abe K. Kamihara K. Katsuta N. Sato K. Tanikawa M. Yamazaki M. Ninomiya K. Ishibashi T. Yamashita H. Murakawa K. Fujimori K. Tanai H. Kimata M. Watanabe M. Hiraoka S. Chiba Y. Ishida S. Ono Y. Takiguchi S. Watanabe S. Yosida M. Hotuta T. Kusano J. Kanehori K. Takahashi-Fujii A. Hara H. Tanase T. -O. Nomura Y. Togiya S. Komai F. Hara R. Takeuchi K. Arita M. Imose N. Musashino K. Yuuki H. Oshima A. Sasaki N. Aotsuka S. Yoshikawa Y. Matsunawa H. Ichihara T. Shiohata N. Sano S. Moriya S. Momiyama H. Satoh N. Takami S. Terashima Y. Suzuki O. Nakagawa S. Senoh A. Mizoguchi H. Goto Y. Shimizu F. Wakebe H. Hishigaki H. Watanabe T. Sugiyama A. Takemoto M. Kawakami B. Yamazaki M. Watanabe K. Kumagai A. Itakura S. Fukuzumi Y. Fujimori Y. Komiyama M. Tashiro H. Tanigami A. Fujiwara T. Ono T. Yamada K. Fujii Y. Ozaki K. Hirao M. Ohmori Y. Kawabata A. Hikiji T. Kobatake N. Inagaki H. Ikema Y. Okamoto S. Okitani R. Kawakami T. Noguchi S. Itoh T. Shigeta K. Senba T. Matsumura K. Nakajima Y. Mizuno T. Morinaga M. Sasaki M. Togashi T. Oyama M. Hata H. Watanabe M. Komatsu T. Mizushima-Sugano J. Satoh T. Shirai Y. Takahashi Y. Nakagawa K. Okumura K. Nagase T. Nomura N. Kikuchi H. Masuho Y. Yamashita R. Nakai K. Yada T. Nakamura Y. Ohara O. Isogai T. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] VARIANT GLY-570. Tissue: Heart. [4] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung. [5] \" A G--> A substitution in an HNF I binding site in the human alpha-fetoprotein gene is associated with hereditary persistence of alpha-fetoprotein (HPAFP). \" McVey J. H. Michaelides K. Hansen L. P. Ferguson-Smith M. Tilghman S. Krumlauf R. Tuddenham E. G. D. Hum. Mol. Genet. 2:379-384(1993) [PubMed: 7684942] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28. [6] Lubec G. Vishwanath V. Submitted (MAR-2007) to UniProtKBCited for: PROTEIN SEQUENCE OF 311-332; 348-372 AND 422-437 MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell. [7] \" Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA. \" Beattie W. G. Dugaiczyk A. Gene 20:415-422(1982) [PubMed: 6187626] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 429-556. [8] \" Human alpha-fetoprotein primary structure: a mass spectrometric study. \" Pucci P. Siciliano R. Malorni A. Marino G. Tecce M. F. Ceccarini C. Terrana B. Biochemistry 30:5061-5066(1991) [PubMed: 1709810] [Abstract]Cited for: PARTIAL PROTEIN SEQUENCE OF 19-609. [9] \" Studies on human alpha-fetoprotein. Isolation and characterization of monomeric and polymeric forms and amino-terminal sequence analysis. \" Yachnin S. Hsu R. Heinrikson R. L. Miller J. B. Biochim. Biophys. Acta 493:418-428(1977) [PubMed: 70228] [Abstract]Cited for: PRELIMINARY PROTEIN SEQUENCE OF 19-35. [10] \" Comparative chemical structures of human alpha-fetoproteins from fetal serum and from ascites fluid of a patient with hepatoma. \" Aoyagi Y. Ikenaka T. Ichida F. Cancer Res. 37:3663-3667(1977) [PubMed: 71198] [Abstract]Cited for: PRELIMINARY PROTEIN SEQUENCE OF 19-38.