MOUSE ANTI V5-TAG

V5-TAG

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.

Has been used to detect recombinant proteins some of which include transmembrane and secreted proteins which have been tagged with this epitope. Usually a 14 amino acid tag has been added to the recombinant proteins although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) 1. Pietravalle F. et al. (1996) Human native soluble CD40L is a biologically active trimer processed inside microsomes. J. Biol. Chem. 271: 5965-5967. [1] \" Cloning of TRAP a ligand for CD40 on human T cells. \" Graf D. Korthaeuer U. Mages H. W. Senger G. Kroczek R. A. Eur. J. Immunol. 22:3191-3194(1992) [PubMed: 1280226] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" The human T cell antigen gp39 a member of the TNF gene family is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity. \" Hollenbaugh D. Grosmaire L. S. Kullas C. D. Chalupny J. N. Braesch-Andersen S. Noelle R. J. Stamenkovic I. Ledbetter J. A. Aruffo A. EMBO J. 11:4313-4321(1992) [PubMed: 1385114] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [3] \" The CD40 ligand gp39 is defective in activated T cells from patients with X-linked hyper-IgM syndrome. \" Aruffo A. Farrington M. Hollenbaugh D. Li X. Milatovich A. Nonoyama S. Bajorath J. Grosmaire L. S. Stenkamp R. Neubauer M. Roberts R. L. Noelle R. J. Ledbetter J. A. Francke U. Ochs H. D. Cell 72:291-300(1993) [PubMed: 7678782] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] VARIANTS HIGM1 128-ARG-GLY-129 AND PRO-235. [4] \" Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. \" Spriggs M. K. Armitage R. J. Strockbine L. Clifford K. N. Macduff B. M. Sato T. A. Maliszewski C. R. Fanslow W. C. J. Exp. Med. 176:1543-1550(1992) [PubMed: 1281209] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [5] \" Human CD40-ligand: molecular cloning cellular distribution and regulation of expression by factors controlling IgE production. \" Gauchat J. -F. Aubry J. -P. Mazzei G. J. Life P. Jomotte T. Elson G. Bonnefoy J. -Y. FEBS Lett. 315:259-266(1993) [PubMed: 7678552] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [6] \" Structural organization of the gene for CD40 ligand: molecular analysis for diagnosis of X-linked hyper-IgM syndrome. \" Shimadzu M. Nunoi H. Terasaki H. Ninomiya R. Iwata M. Kanegasaka S. Matsuda I. Biochim. Biophys. Acta 1260:67-72(1995) [PubMed: 7999797] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [7] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood. [8] \" Human native soluble CD40L is a biologically active trimer processed inside microsomes. \" Pietravalle F. Lecoanet-Henchoz S. Blasey H. Aubry J. -P. Elson G. Edgerton M. D. Bonnefoy J. -Y. Gauchat J. -F. J. Biol. Chem. 271:5965-5967(1996) [PubMed: 8626375] [Abstract]Cited for: PROTEIN SEQUENCE OF 113-117 PROTEOLYTIC PROCESSING. [9] \" Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells. \" Khandekar S. S. Silverman C. Wells-Marani J. Bacon A. M. Birrell H. Brigham-Burke M. DeMarini D. J. Jonak Z. L. Camilleri P. Fishman-Lobell J. Protein Expr. Purif. 23:301-310(2001) [PubMed: 11676606] [Abstract]Cited for: STRUCTURE OF CARBOHYDRATE ON ASN-240 MASS SPECTROMETRY. [10] \" Release of soluble CD40L from platelets is regulated by glycoprotein IIb/IIIa and actin polymerization. \" Furman M. I. Krueger L. A. Linden M. D. Barnard M. R. Frelinger A. L. III Michelson A. D. J. Am. Coll. Cardiol. 43:2319-2325(2004) [PubMed: 15193700] [Abstract]Cited for: FUNCTION.