« Back
Home /
TGFBR2 Knockout 293T Cell Line, Heterozygous

TGFBR2 Knockout 293T Cell Line, Heterozygous

Manufacturer	Abclonal
Category	Cell Lines & Cell Culture Cell Lines
Specific against	other
Amount	1 Strips
Item no.	RM02142-1Strips
Available
protein size	TGFBR2
GeneID(Human)	7048
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Imminogen	Dry ice
Storage	Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
BackGround	This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]
RecommandDilutionA	1 vial parental cell line and 1 vial knockout cell line
ProteinWeight	TGFBR2
AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition. 1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1 Strips

available

Delivery expected until 6/22/2023