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SUMO1 Knockout 293T Cell Line, Homozygous

SUMO1 Knockout 293T Cell Line, Homozygous

Manufacturer	Abclonal
Category	Cell Lines & Cell Culture Cell Lines
Specific against	other
Amount	1 Strips
Item no.	RM02171-1Strips
Available
protein size	SUMO1
GeneID(Human)	7341
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Imminogen	Dry ice
Storage	Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
BackGround	This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]
RecommandDilutionA	1 vial parental cell line and 1 vial knockout cell line
ProteinWeight	SUMO1
AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition. 1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.

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Amount: 1 Strips

available

Delivery expected until 7/27/2023