Comparison

ARG1 Knockout HeLa Cell Line, Homozygous

Manufacturer Abclonal
Category
Type Cell line
Specific against other
Amount 1 Strips
Item no. RM02193-1Strips
eClass 6.1 32160890
eClass 9.0 32160890
Available
AntigenSeq
Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO2 condition.
1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
        dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37℃ incubator with 5% CO2.
7. A subcultivation ratio of 1:2-1:4 is recommended.
BackGround
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
GeneID(Human)
383
Imminogen
Dry ice
ProteinWeight
ARG1
RecommandDilutionA
1 vial parental cell line and 1 vial knockout cell line
Route
Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Storage
Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
protein size
ARG1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 Strips
Available: In stock
available

Delivery expected until 4/30/2024 

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