Comparison

SMPD1 Knockout HeLa Cell Line, Homozygous

Manufacturer Abclonal
Category
Specific against other
Amount 1 Strips
Item no. RM02211-1Strips
Available
protein size
SMPD1
GeneID(Human)
6609
Route
Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Imminogen
Dry ice
Storage
Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
BackGround
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
RecommandDilutionA
1 vial parental cell line and 1 vial knockout cell line
ProteinWeight
SMPD1
AntigenSeq
Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO2 condition.
1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
        dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37℃ incubator with 5% CO2.
7. A subcultivation ratio of 1:2-1:4 is recommended.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 Strips
Available: In stock
available

Delivery expected until 6/22/2023 

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