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DPYSL2 Knockout HeLa Cell Line, Homozygous

DPYSL2 Knockout HeLa Cell Line, Homozygous

Manufacturer	Abclonal
Category	Cell Lines & Cell Culture Cell Lines
Specific against	other
Amount	1 Strips
Item no.	RM02242-1Strips
Available
protein size	DPYSL2
GeneID(Human)	1808
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Imminogen	Dry ice
Storage	Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
BackGround	This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer' s disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
RecommandDilutionA	1 vial parental cell line and 1 vial knockout cell line
ProteinWeight	DPYSL2
AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition. 1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.

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Amount: 1 Strips

available

Delivery expected until 6/22/2023