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CAST Knockout HeLa Cell Lysate

CAST Knockout HeLa Cell Lysate

Manufacturer	Abclonal
Category	Cell Lines & Cell Culture Cell Lysates
Specific against	other
Applications	WB
Amount	100 ug
Item no.	RM02374-100ug
Available
protein size	CAST
GeneID(Human)	831
Route	CAST KO cell lysate and WT cell lysate.
Imminogen	4℃
Storage	Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
BackGround	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
RecommandDilutionA	1 vial parental cell Lysate and 1 vial knockout cell Lysate
ProteinWeight	CAST
AntigenSeq	To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Lysate should be boiled for 3 - 5 minutes before loading onto gel.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

available

Delivery expected until 7/27/2023