Comparison

OPRM1 Knockout 293T Cell Line, Homozygous knockout

Manufacturer Abclonal
Category
Type Cell line
Specific against other
Amount 1 Strips
Item no. RM02447-1Strips
eClass 6.1 32160890
eClass 9.0 32160890
Available
AntigenSeq
Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO2 condition.
1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
        dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37℃ incubator with 5% CO2.
7. A subcultivation ratio of 1:2-1:4 is recommended.
BackGround
This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A> G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains. [provided by RefSeq, Oct 2013]
GeneID(Human)
4988
Imminogen
Dry ice
ProteinWeight
OPRM1
RecommandDilutionA
1 vial parental cell line and 1 vial knockout cell line
Route
Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Storage
Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
protein size
OPRM1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 Strips
Available: In stock
available

Delivery expected until 4/30/2024 

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close