Comparison

Active Recombinant Human IGFBP-3 Protein

Manufacturer Abclonal
Category
Type Proteins Recombinant
Specific against other
Amount 20 ug
Item no. RP01363-20ug
eClass 6.1 34160400
eClass 9.0 42020190
Available
AntigenSeq
GASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK
BackGround
The Insulin-like Growth Factor (IGF) signaling system plays a central role in cellular growth, differentiation, and proliferation. IGFBP3 is the most abundant IGF binding protein in human serum and is a growth inhibitory, apoptosis-inducing molecule, capable of acting via IGF-dependent and IGF-independent mechanisms. It appears to function both by cell cycle blockade and the induction of apoptosis. IGFBP3 can be transported to the nucleus by an importin beta mediated mechanism, where it has been shown to interact with the retinoid X receptor alpha and possibly other nuclear elements. IGFBP3 antiproliferative signaling appears to require an active transforming growth factor-beta (TGF-beta) signaling pathway, and IGFBP3 stimulates phosphorylation of the TGF-beta signaling intermediates Smad2 and Smad3. IGFBP3 has IGF-independent roles in inhibiting cell proliferation in cancer cell lines. Nuclear transcription factor, retinoid X receptor (RXR)-alpha, and IGFBP3 functionally interact to reduce prostate tumor growth and prostate-specific antigen in vivo. Several clinical studies have proposed that individuals with IGFBP3 levels in the upper range of normal may have a decreased risk for certain common cancers. This includes evidence of a protective effect against breast cancer, prostate cancer, colorectal cancer, and lung cancer. Moreover, IGFBP3 inhibits insulin-stimulated glucose uptake into adipocytes independent of IGF.
Cross-Reactivity
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
GeneID(Human)
IGFBP3
GeneSymbol
IGFBP3
Imminogen
Gly28-Lys291
ProteinBioActivity
Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP3 at 1 μg/mL (100 μL/well) can bind Human IGF1 with a linear range of 0.0724-138 ng/mL.
ProteinDescription
Active Recombinant Human IGFBP-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly28-Lys291) of human IGFBP3/BP-53 (Accession #NP_000589.2) fused with a 6×His tag at the C-terminus.
ProteinFormulation
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
RecommandDilutionA
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Route
C-His
Storage
Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
available

Delivery expected until 4/30/2024 

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