« Back
Home /
Human UCH-L1 Protein, His Tag

Human UCH-L1 Protein, His Tag

Item no.	UC1-H5140-50ug
Manufacturer	ACROBiosystems
Amount	50 ug
Quantity options	500 ug x 2 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	E.coli
Conjugate/Tag	Unconjugated
Purity	95%
Citations	(1) Choi J., et al., 2004, J. Biol. Chem. 279:13256-13264. (2) Liu Y., et al., 2002, Cell 111:209-218. (3) Choudhary C., et al., 2009, Science 325:834-840.
NCBI	NP_004172
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	UCHL1,PGP9.5
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	N-6xHis, Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	25.7 kDa
Manufacturer - Format	Powder
Description	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) , a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also, UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
Background	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) , a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also, UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
Molecule	UCH-L1
Exp Region	Gln 2 - Ala 223
Characteristics	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 27-28 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin	1.0 EU per μg
Buffer	50 mM Tris, 150 mM Nacl, pH8.0
Stability	● -20°C to -70°C for 12 months in lyophilized state ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

UC1-H5140-50ug-datasheet.pdf

UC1-H5140-50ug-safety-datasheet.pdf