Recombinant Human Insulin-like Growth Factor-binding Protein-like 1/IGFBPL1 Protein

Proteins|Recombinant Proteins|Human Proteins

Ambient temperature

Recombinant human IGFBPL1 protein has a calculated molecular mass of 26 kDa. Due to the glycosylation sites, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

12352202

Leu26-Met278

Lyophilized powder

Determined by BCA protein assay

Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.