Comparison

Human C1 inhibitor HEK293 Overexpression Lysate European Partner

Manufacturer Sino Biological
Category
Type Cell Lysate
Specific against Human
Format Human Cells
Amount 300ug
Host Human
Item no. 10995-H08HL-300
eClass 6.1 32190290
eClass 9.0 32190290
Available
Molecule
SERPING1
Product Description
Human Cells transfected lysate in which Human SERPING1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Expressed Host
Human Cells
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200uL pure water and boiled for 2-5 min. 3. Store it at -80C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. Notes: The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer
Storage Instruction
Store at -80C. Aliquot to avoid repeated freezing and thawing
Stability
Samples are stable for up to twelve months from date of receipt at -80C
Application notes
WB: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 300ug
Available: In stock
available

Delivery expected until 4/18/2024 

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