Comparison

ELISA Kit for Carbonic Anhydrase VI (CA6)

Manufacturer Cloud-Clone
Category
Type Elisa-Kit
Specific against Human
Applications ELISA
Amount 96T
Item no. SED073Hu
eClass 6.1 32160605
eClass 9.0 32160605
Available
Target Name
Carbonic Anhydrase VI
Alternative Names
CA-VI, CAVI, GUSTIN, Carbonate dehydratase VI, Salivary carbonic anhydrase, Secreted carbonic anhydrase
Uniprot
P23280
Detection range
0.312-20ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.112ng/mL
Sample type
Serum, plasma, saliva and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase VI (CA6).
No significant cross-reactivity or interference between Carbonic Anhydrase VI (CA6) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VI (CA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VI (CA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VI (CA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase VI (CA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Enzyme & Kinase,
References
Salivary markers of taste sensitivity to oleic acid: a combined proteomics and metabolomics approach
SALIVARY CARBONIC ANHYDRASE VI, ZINC SULFATE TASTE ACUITY AND FREQUENCY OF ILLNESS: A PILOT STUDY
Associations between food consumption patterns and saliva composition: Specificities of eating difficulties children.
Differences in the density of fungiform papillae and composition of saliva in patients with taste disorders compared to healthy controls
Children with a Higher Activity of Carbonic Anhydrase VI in Saliva Are More Likely to Develop Dental Caries.
Obese Subjects With Specific Gustatory Papillae Microbiota and Salivary Cues Display an Impairment to Sense Lipids
Acceptance of added fat to first complementary feeding purees: An exploration of fat type, feeding history and saliva composition
Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

Amount: 96T
Available: In stock
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