Protein Name & Synonyms:
LETM1
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Mouse
LETM1 antibody, "LETM1 and EF-hand domain-containing protein 1, mitochondrial antibody", leucine zipper-EF-hand-containing transmembrane protein 1 antibody, Mdm38 homolog antibody, leucine zipper-EF-hand containing transmembrane protein 1 antibody
Species Reactivity
Human, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
83 kDa
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 506 and 723 (O95202) of LETM1
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using LETM1(107-11028) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using LETM1(107-11028) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-11028 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
D: Hep G2
7.5% SDS PAGE
107-11028 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.