Protein Name & Synonyms:
DLST
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human
DLTS antibody, DLST antibody, E2K antibody, "dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial antibody", OGDC-E2 antibody, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) antibody
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
49 kDa
Recombinant fragment corresponding to a region within amino acids 188 and 453 of DLST (Uniprot ID# P36957)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using DLST(107-11032) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DLST(107-11032) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using DLST(107-11032) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-11032 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.