Protein Name & Synonyms:
RPA32
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Rat, Mouse
REPA2 antibody, RPA32 antibody, RPA2 antibody, RP-A p34 antibody, replication protein A 32 kDa subunit antibody, RP-A p32 antibody, RF-A protein 2 antibody, replication protein A 34 kDa subunit antibody, replication factor A protein 2 antibody, replication protein A2, 32kDa antibody
Species Reactivity
Human, Rat, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 202 of RPA32 (Uniprot ID# P15927)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (107-11044) dilution: 1:1000
RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (107-11044) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 32 kDa subunit (107-11044) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (107-11044) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
107-11044 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.