Protein Name & Synonyms:
VCP, N-term
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Rat, Mouse
IBMPFD antibody, MGC131997 antibody, MGC148092 antibody, MGC8560 antibody, TERA antibody, p97 antibody, VCP antibody, yeast Cdc48p homolog antibody, 15S Mg(2+)-ATPase p97 subunit antibody, TER ATPase antibody, transitional endoplasmic reticulum ATPase antibody, valosin-containing protein antibody, valosin containing protein antibody
Species Reactivity
Human, Rat, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
89 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID# P55072)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
107-11045 diluted at 1:5000
VCP antibody detects VCP protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (107-11045) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(107-11045) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(107-11045) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
C: Molt-4
D: Raji
7.5% SDS PAGE
107-11045 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.