Protein Name & Synonyms:
RACGAP1, N-term
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Mouse
HsCYK-4 antibody, ID-GAP antibody, MgcRacGAP antibody, RACGAP1 antibody, male germ cell RacGap antibody, rac GTPase-activating protein 1 antibody, protein CYK4 homolg antibody, GTPase activating protein antibody, Rac GTPase activating protein 1 antibody
Species Reactivity
Human, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
71 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 202 of RACGAP1 (Uniprot ID# Q9H0H5)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1(107-11052) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1(107-11052) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
107-11052 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using RACGAP1(107-11052) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
107-11052 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.