Protein Name & Synonyms:
FANCA, N-term
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human
FA antibody, FA-H antibody, FA1 antibody, FAA antibody, FACA antibody, FAH antibody, FANCH antibody, MGC75158 antibody, FANCA antibody, "Fanconi anemia, complementation group H antibody", Fanconi anemia group A protein antibody, "Fanconi anemia, type 1 antibody", "Fanconi anemia, complementation group A antibody"
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
163 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 220 of FANCA (Uniprot ID# O15360)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FACA(107-11063) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded OV90 xenograft, using FACA(107-11063) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: Hela
12% SDS PAGE
107-11063 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.