Protein Name & Synonyms:
MVP/LRP
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Mouse
LRP antibody, VAULT1 antibody, MVP antibody, lung resistance-related protein antibody, major vault protein antibody
Species Reactivity
Human, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
99 kDa
Recombinant fragment corresponding to a region within amino acids 158 and 380 of MVP/LRP (Uniprot ID# Q14764)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MVP/LRP(107-11081) antibody at 1:200 dilution.
MVP/LRP antibody detects MVP/LRP protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MVP/LRP antibody (107-11081) dilution: 1:500.
MVP/LRP antibody detects MVP protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
7.5 % SDS-PAGE
MVP/LRP antibody (107-11081) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LRP(MVP)(107-11081) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-11081 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.