Protein Name & Synonyms:
TRIM32
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Concentration:
0.74 mg/ml
Species Reactivity:
Human, Rat
BBS11 antibody, HT2A antibody, LGMD2H antibody, TATIP antibody, TRIM32 antibody, zinc-finger protein HT2A antibody, 72 kDa Tat-interacting protein antibody, tripartite motif-containing 32 antibody, tripartite motif-containing protein 32 antibody, zinc finger protein HT2A antibody, TAT-interactive protein, 72-KD antibody, E3 ubiquitin-protein ligase TRIM32 antibody, tripartite motif containing 32 antibody
Species Reactivity
Human, Rat
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
72 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 289 of TRIM32 (Uniprot ID# Q13049)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in ice-cold MeOH for 5 min.
Green: TRIM32 protein stained by TRIM32 antibody (107-11099) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 30 ?g 293T whole cell lysate/extract
7.5 % SDS-PAGE
TRIM32 antibody (107-11099) dilution: 1:1000
TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 5 ?g 293T whole cell lysate/extract
B. 5 ?g whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells
7.5 % SDS-PAGE
TRIM32 antibody (107-11099) dilution: 1:20000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.