Protein Name & Synonyms:
hnRNP F
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Concentration:
0.72 mg/ml
Species Reactivity:
Human
HNRPF antibody, MGC110997 antibody, OK/SW-cl.23 antibody, mcs94-1 antibody, HNRNPF antibody, HnRNP F protein antibody, heterogeneous nuclear ribonucleoprotein F antibody, nucleolin-like protein mcs94-1 antibody
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
46 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 204 of hnRNP F (Uniprot ID# P52597)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F (107-11123) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft , using hnRNP F (107-11123) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
107-11123 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.