Protein Name & Synonyms:
Ladinin 1
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human
LadA antibody, MGC10355 antibody, LAD1 antibody, linear IgA disease antigen homolog antibody, lad-1 antibody, ladinin-1 antibody, linear IgA bullous dermatosis antigen antibody, ladinin 1 antibody
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
57 kDa
Recombinant fragment corresponding to a region within amino acids 300 and 517 of Ladinin 1 (Uniprot ID# O00515)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using Ladinin 1(107-11126) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Colon ca, using Ladinin 1(107-11126) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
107-11126 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.