Protein Name & Synonyms:
PCBP2
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Mouse
HNRPE2 antibody, MGC110998 antibody, hnRNP-E2 antibody, PCBP2 antibody, heterogenous nuclear ribonucleoprotein E2 antibody, heterogeneous nuclear ribonucleoprotein E2 antibody, hnRNP E2 antibody, poly(rC)-binding protein 2 antibody, alpha-CP2 antibody, poly(rC) binding protein 2 antibody
Species Reactivity
Human, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
39 kDa
Recombinant fragment corresponding to a region within amino acids 1 and 193 of PCBP2 (Uniprot ID# Q15366)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using PCBP2(107-11131) antibody at 1:200 dilution.
PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PCBP2 antibody [N1C1] (107-11131) dilution: 1:500.
PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PCBP2 antibody [N1C1] (107-11131) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-11131 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
107-11131 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.