Protein Name & Synonyms:
ACOX3
Applications:
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human, Mouse
ACOX3 antibody, pristanoyl-CoA oxidase antibody, peroxisomal acyl-coenzyme A oxidase 3 antibody, acyl-Coenzyme A oxidase 3, pristanoyl antibody, branched-chain acyl-CoA oxidase antibody, BRCACox antibody, acyl-CoA oxidase 3, pristanoyl antibody
Species Reactivity
Human, Mouse
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
78 kDa
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 408 and 603 (O15254) of ACOX3
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using ACOX3(107-11151) antibody at 1:500 dilution.
ACOX3 antibody detects ACOX3 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
7.5 % SDS-PAGE
ACOX3 antibody (107-11151) dilution: 1:500
Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
107-11151 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.