Protein Name & Synonyms:
KYNU, Internal
Applications:
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Concentration:
0.63 mg/ml
Species Reactivity:
Human
KYNU antibody, kynureninase (L-kynurenine hydrolase) antibody, kynureninase antibody
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Recombinant fragment corresponding to a region within amino acids 114 and 329 of KYNU (Uniprot ID# Q16719)
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using KYNU(107-11156) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
107-11156 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.