Protein Name & Synonyms:
Homer3
Applications:
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Species Reactivity:
Human
HOMER-3 antibody, VESL3 antibody, HOMER3 antibody, "Homer, neuronal immediate early gene, 3 antibody", homer protein homolog 3 antibody, homer homolog 3 (Drosophila) antibody
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
40 kDa
Recombinant fragment corresponding to a region within amino acids 55 and 305 of Homer3 (Uniprot ID# Q9NSC5)
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using Homer3(107-11159) antibody at 1:500 dilution.
Homer3 antibody detects Homer3 protein at cytoplasm on Papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Papillary adenocarcinoma .
Homer3 antibody (107-11159) dilution: 1:250.
Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
107-11159 diluted at 1:1000
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.