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Collagen Hybridizing Peptide, Cy3 Conjugate

Hersteller 3Helix
Kategorie
Typ Peptides
Specific against other
Menge 300ug
ArtNr HEL-RED300
Konjugat/Tag Cy3
eClass 6.1 34160400
eClass 9.0 32160409
Lieferbar
Description
The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1, 2, 3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.
CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[7]
R-CHP is labeled with sulfo-Cyanine3 for direct fluorescence detection.
Applications info
Immunofluorescence, cell imaging, SDS-PAGE (in-gel western)
Features
More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
A non-antibody approach with no species restrictions against any co-staining antibody
Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
Stable in solution under 4 C, eliminating the need to aliquot for storage
Key Publications
Targeting and mimicking collagens via triple helical peptide assemblies. Curr. Opin. Chem. Biol., 2013.
Targeting collagen strands by photo-triggered triple-helix hybridization. Proc. Natl. Acad. Sci. U.S.A., 2012.
In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano, 2017.
Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging. Biomaterials, 2018.
Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nat. Commun., 2017.
Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide. Acta Biomater., 2017.
Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides. Bioconjug. Chem., 2013.
Product Citations
Jeffery Molkentin et al., An acute immune response underlies the benefit of cardiac adult stem cell therapy. bioRxiv (2019)
Xudong Li et al., Molecular detection and assessment of intervertebral disc degeneration via a collagen hybridizing peptide. ACS Biomaterials Science & Engineering (2019)
Xudong Li et al., Microfluidic disc-on-a-chip device for mouse intervertebral disc - pitching a next-generation research platform to study disc degeneration. ACS Biomaterials Science & Engineering (2019)
Karl Kadler et al., Protection of circadian rhythms by the protein folding chaperone, BiP. The FASEB Journal (2019)
Stephen Weiss et al., Divergent matrix-remodeling strategies distinguish developmental from neoplastic mammary epithelial cell invasion programs. Developmental Cell (2018)
Fiona Watt et al., Fibroblast state switching orchestrates dermal maturation and wound healing. Molecular Systems Biology (2018)
Fiona Watt et al., Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation. Plos One (2018)
Matthew Abramowitz et al., Skeletal muscle fibrosis is associated with decreased muscle inflammation and weakness in patients with chronic kidney disease. American Journal of Physiology-Renal Physiology (2018)
Kenneth Monson et al., Detection and characterization of molecular-level collagen damage in overstretched cerebral arteries. Acta Biomaterialia (2018)
Spencer Szczesny et al., Fatigue loading of tendon results in collagen kinking and denaturation but does not change local tissue mechanics. Journal of Biomechanics (2018)
Samuel Veres et al., In tendons, differing physiological requirements lead to functionally distinct nanostructures. Scientific Reports (2018)
Svenja Illien-Junger et al., Dietary advanced glycation end-product consumption leads to mechanical stiffening of murine intervertebral discs. Disease Models & Mechanisms (2018)
Mark Banaszak Holl et al., Fatigue failure mechanism of anterior cruciate ligament fracture. (2018)
Themis Kyriakides et al., Decellularized materials derived from TSP2-KO mice promote enhanced neovascularization and integration in diabetic wounds. Biomaterials (2018)
Per Fogelstrand et al., Systematic in vitro comparison of decellularization protocols for blood vessels. Plos One (2018)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 300ug
Lieferbar: In stock
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Lieferung vsl. bis 02.05.2024 

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