Hersteller |
Abbkine Scientific
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Human |
Applikationen |
ELISA |
Menge |
48T |
ArtNr |
ABK-KTE61908-48T |
Targets |
KLC1 |
eClass 6.1 |
32160605 |
eClass 9.0 |
32160605 |
Lieferbar |
|
Applications notes |
This Human Kinesin light chain 1 (KLC1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLC1 in samples. An antibody specific for KLC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Human Kinesin light chain 1 microplate Human Kinesin light chain 1 standard Human Kinesin light chain 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Human Kinesin light chain 1 (KLC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLC1. No significant cross-reactivity or interference between Human KLC1 and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Kinesin 2 is a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined. |
Gene ID |
3831 |
Alternative |
KLC1; KLC; KNS2; KNS2A; MGC15245; kinesin 2 60/70kDa; medulloblastoma antigen MU-MB-2.50 |
Accession |
Q07866 |
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