Human Duffy antigen/chemokine receptor (DARC) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Duffy antigen/chemokine receptor microplate
Human Duffy antigen/chemokine receptor standard
Human Duffy antigen/chemokine receptor detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Duffy blood group, chemokine receptor (DARC) acts as a non-classical chemokine receptor that does not signal but rather acts as a scavenger protein. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.The Duffy system enjoys the distinction of being the first blood group whose genetic locus was assigned to a specific autosome, i.e., chromosome 1. On the basis of families studied in Rochester, N.Y., Weitkamp (1972) could demonstrate no linkage of the HBB locus and Duffy, as had been suggested by Nance et al. (1970). An earlier suspicion of localization to chromosome 16 was apparently in error. Duffy and the locus for a form of hereditary cataract are closely linked. From extensive family studies, Robson et al. (1973) arrived at a tentative map of chromosome 1.

DARC; CTA-134P22.3; CCBP1; CD234; Dfy; FY; GPD; GpFy; WBCQ1; Duffy blood group antigen; Fy glycoprotein; OTTHUMP00000035293; OTTHUMP00000035294; glycoprotein D