Hersteller |
Abbkine Scientific
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Human |
Applikationen |
ELISA |
Menge |
96Tx50 |
ArtNr |
ABK-KTE62507-96Tx50 |
eClass 6.1 |
32160605 |
eClass 9.0 |
32160605 |
Lieferbar |
|
Applications notes |
This Human Homocysteine (HCY) ELISA Kit employs a two-site sandwich ELISA to quantitate Hcy in samples. An antibody specific for Hcy has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHcy present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hcy is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hcy bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Human Homocysteine microplate Human Homocysteine standard Human Homocysteine detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Human Homocysteine (HCY) ELISA Kit has high sensitivity and excellent specificity for detection of Human Hcy. No significant cross-reactivity or interference between Human Hcy and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Homocysteine is an amino acid with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the amino acid cysteine, differing by an additional methylene (-CH2-) group. It is biosynthesized from methionine by the removal of its terminal Cepsilon methyl group. Homocysteine can be recycled into methionine or converted into cysteine with the aid of B-vitamins.Homocysteine is not obtained from the diet. Instead, it is biosynthesized from methionine via a multi-step process. First, methionine receives an adenosine group from ATP, a reaction catalyzed by S-Adenosyl-methionine synthetase, to give S-adenosyl methionine ("SAM"). SAM then transfers the methyl group to an acceptor molecule. The adenosine is then hydrolyzed to yield L-homocysteine. L-homocysteine has two primary fates: conversion via tetrahydrofolate (THF) back into L-methionine or conversion to L-cysteine. |
Alternative |
HCY |
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