Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62543-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKBIE in samples. An antibody specific for NFKBIE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFKBIE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKBIE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKBIE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon microplate
Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon standard
Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) ELISA Kit has high sensitivity and excellent specificity for detection of Human NFKBIE. No significant cross-reactivity or interference between Human NFKBIE and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
NFKBIE protein expression is up-regulated following NF-kappaB activation and during myelopoiesis. NFKBIE is able to inhibit NF-kappaB-directed transactivation via cytoplasmic retention of REL proteins.NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-kappaB transcription factor complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex.For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6), AP-1 (JUN), and NFAT (see NFATC1).
Gene ID
4794
Alternative
NFKBIE; IKBE;
Accession
O00221
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 16.839,95 €
Preis: 16.839,95 €
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