Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human DNA microplate
Human DNA standard
Human DNA detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. DNMT3A encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.The mouse genes, termed Dnmt3a and Dnmt3b, show little sequence similarity to mouse Dnmt1 and Dnmt2, and masc1 from Ascobolus. The Dnmt3a cDNA encodes a protein of 908 amino acids. The human DNMT3A and DNMT3B cDNAs are highly homologous to the mouse genes. Dnmt3a and Dnmt3b transcripts were abundantly expressed in undifferentiated embryonic stem cells.

DNMT3A; DNMT3A2; M.HsaIIIA; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase HsaIIIA