Human Complement 1q (C1q) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62580-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Complement 1q (C1q) ELISA Kit employs a two-site sandwich ELISA to quantitate C1QB in samples. An antibody specific for C1QB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC1QB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C1QB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C1QB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Complement 1q microplate
Human Complement 1q standard
Human Complement 1q detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Complement 1q (C1q) ELISA Kit has high sensitivity and excellent specificity for detection of Human C1QB. No significant cross-reactivity or interference between Human C1QB and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
C1q is a 400 KDa protein formed from 18 peptide chains in 3 subunits of 6. Each 6 peptide subunit consists of a Y-shaped pair of triple peptide helices joined at the stem and ending in a globular non-helical head.C1q is alarge molecule made up of 18 polypeptide chain s(6 of A, 6 of B, and 6 of C)held together by acombination of covalent(disulfide) and noncovalent bonds. When assembled, the C1q full molecule has two distinct regions:a globular head and a longtail.Most of the antibodies raised against C1q specific to the secondary structure of the assembled chains and are nonreactive to the amino acid sequence of each chain.The tightly bound individual polypeptide chains are difficult to separate from this complex, which requires long incubations under reducing conditions and high urea concentrations.Several proteins have globular domains similar to C1q.
Gene ID
713
Alternative
C1QB; Complement component 1; q subcomponent; beta polypeptide; complement component C1q; B chain; complement subcomponent C1q chain B
Accession
P02746
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 16.839,95 €
Preis: 16.839,95 €
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