Mouse Thymosin beta-4 (TMSB4X) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70147-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Thymosin beta-4 (TMSB4X) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB4X in samples. An antibody specific for TMSB4X has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB4X present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB4X is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB4X bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Thymosin beta-4 microplate
Mouse Thymosin beta-4 standard
Mouse Thymosin beta-4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Thymosin beta-4 (TMSB4X) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMSB4X. No significant cross-reactivity or interference between Mouse TMSB4X and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
The predominant form of thymosin, thymosin beta4, is a member of a highly conserved family of actin monomer-sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin.
Thymosin beta4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin beta4 is regarded as the principal actin-sequestering protein. Thymosin beta4 binds ATP G- monomeric actin in a 11 complex where G-actin cannot polymerize.
Gene ID
19241
Alternative
TMSB4X; FX; PTMB4; TB4X; TMSB4; OTTHUMP00000022925; OTTHUMP00000022927; OTTHUMP00000022928; prothymosin beta-4; thymosin beta-4; thymosin; beta 4; thymosin; beta 4; X chromosome
Accession
P20065

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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