Mouse Tomoregulin-1 (TMEFF1) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70195-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Tomoregulin-1 (TMEFF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEFF1 in samples. An antibody specific for TMEFF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEFF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Tomoregulin-1 microplate
Mouse Tomoregulin-1 standard
Mouse Tomoregulin-1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Tomoregulin-1 (TMEFF1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEFF1. No significant cross-reactivity or interference between Mouse TMEFF1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
EGF-like domain is an evolutionary conserved protein domain, of about thirty to forty amino-acid residues long, which was found in a large number of mostly animal proteins.Eib and Martens (1996) cloned a novel Xenopus gene, 7365, that is enriched in neuroendocrine tissues. The predicted protein contains a signal peptide, a transmembrane domain, 2 follistatin modules, and an EGF-like domain, a cysteine-rich region first identified in epidermal growth factor. Eib et al. (1998) stated that the unique EGF-like domain and transmembrane and cytoplasmic regions of the human homolog are nearly identical to those found in the mouse and frog homologs.Eib et al. (1998) used somatic cell hybrids and fluorescence in situ hybridization to map the TMEFF1 gene to human chromosome 9q31.
Gene ID
230157
Alternative
TMEFF1; C9orf2; H7365; tomoregulin-1
Accession
Q6PFE7

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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