Mouse TM2 domain-containing protein 3 (TM2D3) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70204-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse TM2 domain-containing protein 3 (TM2D3) ELISA Kit employs a two-site sandwich ELISA to quantitate TM2D3 in samples. An antibody specific for TM2D3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM2D3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM2D3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM2D3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse TM2 domain-containing protein 3 microplate
Mouse TM2 domain-containing protein 3 standard
Mouse TM2 domain-containing protein 3 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse TM2 domain-containing protein 3 (TM2D3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TM2D3. No significant cross-reactivity or interference between Mouse TM2D3 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TM2D3 contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily. This protein has sequence and structural similarities to the beta-amyloid binding protein (BBP), but, unlike BBP, it does not regulate a response to beta-amyloid peptide. The deduced 221-amino acid protein contains several features of a G protein-coupled receptor, including 2 putative transmembrane domains in its C-terminal half, a DRY motif, and conserved cysteines and lysines. Northern blot analysis detected variable expression of a 1.4-kb BLP2 transcript in all human tissues examined. In situ hybridization of cynomolgus monkey coronal sections revealed extensive expression in neurons of the hippocampus/entorhinal cortex and neocortical regions.
Gene ID
68634
Alternative
TM2D3; BLP2; BBP-like protein 2
Accession
Q8BJ83
Menge: 48T
Lieferbar: In stock
Listenpreis: 344,37 €
Preis: 344,37 €
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