Mouse Protein spinster homolog 1 (SPNS1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Protein spinster homolog 1 microplate
Mouse Protein spinster homolog 1 standard
Mouse Protein spinster homolog 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker.

SPNS1; FLJ38358; HSpin1; LAT; PP2030; SPIN1; SPINL; nrs; spinster homolog 1; spinster-like