Mouse Protein spinster homolog 1 (SPNS1) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70231-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Protein spinster homolog 1 (SPNS1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPNS1 in samples. An antibody specific for SPNS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPNS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Protein spinster homolog 1 microplate
Mouse Protein spinster homolog 1 standard
Mouse Protein spinster homolog 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Protein spinster homolog 1 (SPNS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPNS1. No significant cross-reactivity or interference between Mouse SPNS1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker.
Gene ID
73658
Alternative
SPNS1; FLJ38358; HSpin1; LAT; PP2030; SPIN1; SPINL; nrs; spinster homolog 1; spinster-like
Accession
Q8R0G7
Menge: 48T
Lieferbar: In stock
Listenpreis: 344,37 €
Preis: 344,37 €
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