Mouse Calcium-independent phospholipase A2 (PLA2G6) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Calcium-independent phospholipase A2 microplate
Mouse Calcium-independent phospholipase A2 standard
Mouse Calcium-independent phospholipase A2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

85 kDa calcium-independent phospholipase A2 is is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date.Tang et al. (1997) purified an 85-kD cytosolic calcium-independent PLA2, termed iPLA2, from Chinese hamster ovary (CHO) cells. They isolated a partial human cDNA from a Burkitt lymphoma cDNA library by screening with a CHO cDNA. Northern blot analysis revealed that iPLA2 is expressed in a variety of rodent tissues.

PLA2G6; CTA-228A9.2; CaI-PLA2; GVI; INAD1; IPLA2-VIA; PARK14; PLA2; PNPLA9; iPLA2; OTTHUMP00000199457; cytosolic; calcium-independent phospholipase A2; patatin-like phosph; CaI-PLA2; GVI; INAD1; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2, group VI(Cytosolic, Calcium-Independent)