Rat Hyaluronan and proteoglycan link protein 1 (HAPLN1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rat Hyaluronan and proteoglycan link protein 1 microplate
Rat Hyaluronan and proteoglycan link protein 1 standard
Rat Hyaluronan and proteoglycan link protein 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Cartilage link protein stabilizes aggregates of aggrecan and hyaluronan, giving cartilage its tensile strength and elasticity. Cartilage provides the template for endochondral ossification and is crucial for determining the length and width of the skeleton. During endochondral bone formation, hypertrophic chondrocytes die and the cartilage is replaced with bone matrix. The deduced amino acid sequence is 354 residues long and shows a striking degree of similarity to porcine, rat, and chicken link protein sequences. By in situ hybridization, they mapped the gene to 5q13-q14.1. CRTL1 gene comprises 5 exons and is spread over more than 60 kb. Primer extension and S1 nuclease protection analysis revealed transcription initiation to be 315 bases upstream from the translation initiation codon.

HAPLN1; CRTL1; Cartilage link protein; cartilage linking protein 1