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PurKine His-Tag Ni-Super Packed Column

PurKine His-Tag Ni-Super Packed Column

Hersteller	Abbkine Scientific
Kategorie	Reagents & Chemicals Nanoparticles Beads & Resin
Typ	Chemicals
Specific against	other
Menge	3mlx5
ArtNr	ABK-BMC20020-3mlx5
eClass 6.1	32160000
eClass 9.0	32160000
Lieferbar
Applications notes	PurKine His-Tag Ni-Super Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90um beads of highly cross-linked 4% agarose, to which Super replacement to NTA has been coupled, and then been charged with nickel ions (Ni2+) closely. This featured chelating group have more stable binding with Nii2+ than NTA, thus featured more resistance to various chemicals including reducing, chelating agents. Tests confirm that no decrease in performance occurs after at least five repeated uses. This His-Tag Ni-Super Resin is suitable for purification of His-tag proteins from samples that can cause Ni stripping from the medium, e.g. secreted proteins in solution containing chelators.
Formulation	Prepacked columns prepacked with 50% slurry in 20% ethanol.
Features & Benefits	High capacity—More than 10 mg of 6xHis-tagged protein per milliliter of resin. High toleration—More tolerable to various chemicals including reducing, chelating agents.The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours in 10 mM EDTA Versatile—For purification under native or denaturing conditions. Cost-saving—No performance decrease after at least five repeated uses of the same batch of resin. Flexible—available in multiple formats including bulk resin, spin columns and complete kits.Easy Cleaning—Directly use NaOH to cleaning-in-place.
Storage buffer	Liquid resin in PBS buffer containing 20% ethanol were prepacked in columns.
Storage instructions	Stable for one year at 2-8C from date of shipment. Do not freeze.
Shipping	Blue ice
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PurKine His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine His-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative	Ni-NTA; His resin; Ni-NTA resin; His tag resin

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 3mlx5

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Lieferung vsl. bis 18.07.2024

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