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PurKine MBP-Tag Dextrin Packed Column 6FF

Hersteller Abbkine Scientific
Kategorie
Typ Chemicals
Specific against other
Menge 3mlx5
ArtNr ABK-BMC20206-3mlx5
eClass 6.1 32160000
eClass 9.0 32160000
Lieferbar
Applications notes
PurKine MBP-Tag Dextrin Resin 6FF effectively purifies high levels of overexpressed MBP-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90um beads of highly cross-linked 6% agarose, to which Dextrin has been coupled. Tests confirm that performance equals or exceeds popular Dextrin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.
Formulation
Prepacked columns prepacked with 50% slurry in 20% ethanol.
Features & Benefits
High capacity—More than 10 mg of MBP-tagged protein per milliliter of resin.
High performance—Physiological conditions and mild elution is performed using maltose which preserve target protein activity.
Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour.
Storage buffer
Liquid resin in PBS buffer containing 20% ethanol were prepacked in columns.
Storage instructions
Stable for one year at 2-8C from date of shipment. Do not freeze.
Shipping
Blue ice
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
PurKine MBP-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purification of recombinant maltose binding proteins (MBP-tagged protein). Recombinant proteins with MBP-tags often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies). The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine MBP-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative
Dextrin ; MBP resin; Dextrin resin; MBP tag resin

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 3mlx5
Lieferbar: In stock
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Lieferung vsl. bis 18.07.2024 

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