Human VEGF-C protein, His Tag

1. Measured by its binding ability in a functional ELISA .
Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM .
2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).
The ED50 for this effect is 0.1-0.5ug/mL.

Human Cells

Lyophilized from sterile PBS, pH 7.4

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human VEGF-C protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The product is shipped at ambient temperature.

Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.

Flt4-L; LMPH1D; VEGF-C; VRP; Vascular endothelial growth factor C; VEGFC

P49767