Beclin-1

Hersteller Abclonal
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse
Applikationen WB, IP, IHC
Protein Familie Apoptosis/Autophagy
Menge 200ug
Host Rabbit
ArtNr A0562-200ug
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
*

DESCRIPTION

Calculated MW
52kDa
Immunogen
Recombinant protein of human Beclin-1
Gene ID
8678
Swiss Prot
Q14457
Synonyms
BECN1; ATG6; VPS30; beclin1;
Isotype
IgG
Purity
Affinity purification
Storage Buffer
Store at -20? or -80?. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). It is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response although responses to apoptosis appeared normal (7). Overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).
Recommended Dilutions
WB 1:200-1:500 IHC 1:50-1:100 IP 1:20-1:50
illustration
Western blot analysis of extracts of A431 cellline, using Beclin-1antibody.
Menge: 200ug
Lieferbar: In stock
Listenpreis: 444,40 €
Preis: 444,40 €
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