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Tylosin (TYL) ELISA Kit

ArtNr ABE-AE81033SF-5x96T
Hersteller Abebio
Menge 5x96T
Kategorie
Typ Elisa-Kit
Specific against other
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Abbreviation
TYL
Range
1.5-121.5 ppb
Sensitivity
1.5 ppb
Sample Type
Tissue, liver, honey, etc.
Detection Method
Competitive ELISA
Analysis Method
Quantitive
Assay Duration
1-3h
Sample Volume
10-200 uL
Detection Wavelengt
450 nm
Availability
3-5 working days
Test principle
This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TYL antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for TYL and Horseradish Peroxidase (HRP) conjugated TYL. The competitive inhibition reaction is launched between with HRP labeled TYL and unlabeled TYL with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TYL in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview
Tylosin is a bacteriostat feed additive used in veterinary medicine. It has a broad spectrum of activity against Gram-positive organisms and a limited range of Gram-negative organisms. It is found naturally as a fermentation product of Streptomyces fradiae. It is a macrolide antibiotic. Tylosin is used in veterinary medicine to treat bacterial infections in a wide range of species and has a high margin of safety. It has also been used as a growth promotant in some species, and as a treatment for colitis in companion animals. Like other macrolides, tylosin has a bacteriostatic effect on susceptible organisms, caused by inhibition of protein synthesis through binding to the 50S subunit of the bacterial ribosome.
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
Storage
Store at 2-8C. Please refer to Instruction Manual.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5x96T
Lieferbar: In stock
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