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Nitroimidazole (metronidazole) ELISA Kit

ArtNr ABE-AE81034SF-48T
Hersteller Abebio
Menge 48T
Kategorie
Typ Elisa-Kit
Specific against other
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Abbreviation
metronidazole
Range
0.05-12.8 ppb
Sensitivity
0.05 ppb
Sample Type
Fish, shrimp, poultry, liver, honey, etc.
Detection Method
Competitive ELISA
Analysis Method
Quantitive
Assay Duration
1-3h
Sample Volume
10-200 uL
Detection Wavelengt
450 nm
Availability
3-5 working days
Test principle
This assay is based on the competitive enzyme immunoassay for the detection of metronidazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-metronidazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the metronidazole in it. This value is compared to the standard curve and the metronidazole concentration is subsequently obtained.
Product Overview
5-Nitroimidazole is an imidazole derivative that contains a nitro group. Several derivatives of nitroimidazole constitute the class of nitroimidazole antibiotics that have been used to combat anaerobic bacterial and parasitic infections. Perhaps the most common example is metronidazole (Flagyl). Other heterocycles such as nitrothiazoles (thiazole) are also used for this purpose. Nitroheterocycles may be reductively activated in hypoxic cells, and then undergo redox recycling or decompose to toxic products.
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
Storage
Store at 2-8C. Please refer to Instruction Manual.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48T
Lieferbar: In stock
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