AceSeq FFPE DNA Purification Kit

AceSeq FFPE RNA Purification Kit is designed for quick isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples as little as 1 hour. The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.




The protocol of AceSeq FFPE DNA Extraction Kit requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR. AcceGen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping.