A-72 cells

Disease:

Tumor

Origin:

Derived from tumour of thigh (unknown histology) of 8 year old Golden Retriever which had a previous history of mammary adenocarcinoma. Susceptible to canine corona-, canine Ad type I and II, canine herpes, canine parainfluenza and canine parvo- viruses. A canine parvovirus related to Feline panleukopenia virus has been recovered from A-72 cells. Use for efficacy testing.

Species:

Canis familiari, dog

Tissue:

Unknown

Morphology:

Fibroblast, fibroblast-like

Properties:

Adherent

Patient:

Female, 8 years of age

Medium:

AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) , or equivalent medium + 10% FBS (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculture:

When 70-80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:8; feed cells 2 to 3 times weekly. Culture at 100% Air (or tightly close the cap), 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO